Generator
WOG
Part:BBa_J45120
Designed by: MIT IGEM 2006 Group: iGEM06_MIT (2006-08-10)
Wintergreen odor generator
The wintergreen odor generator (BBa_J45120) catalyzes the conversion of the precursor salicylic acid to the odor methyl salicylate that has a wintergreen smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0040) and an odor enzyme generator (BBa_J45119). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.
Usage and Biology
- Supplement cultures with 2 mM salicylic acid to produce wintergreen odor.
- See BBa_J45004 for details.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 744
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 285
[edit]
Categories
Parameters
//classic/generator/prct
//function/odor
s
//function/odor
s
o_h | |
protein | BSMT1 |
rbs | 149 |
tag | None |